Transepithelial migration

Cell migration is the movement of cells from one location to another, usually in response to a chemical signal. It is key for functions such as wound repair, embryonic development and metastasis. Cell invasion is similar to cell migration - however it requires the passage of cells through extracellular matrix or basement membrane extract and is key for processes such as inflammation and metastasis.

A range of transwell inserts are available to assess cell migration and invasion. The principles are based on the Boyden chamber which consists of a chamber of two medium-filled compartments separated by a microporous membrane. In general, cells are placed in the upper compartment and are allowed to migrate through the pores of the membrane into the lower compartment, in which chemotactic agents are present. The size of the motile cells to be investigated, determine the pore size of the filter; it is essential to choose a diameter which allows an active transmigration. After an appropriate incubation time, the number of cells that have migrated to the lower side of the membrane is determined.

Serum batch/medium testing

We are able to test new batches of serum, reagents, medium or any other cell culture based component, providing independent feedback and saving you precious time. Our team is experienced in culturing both cell lines and primary cells, thus providing a wide range of services. Please contact us for a customised testing protocol and quote.